ANTIMICROBIAL PROPERTIES OF TRACE PHYTOCHEMICAL COMPOUNDS ANALYSIS OF Azadirachta indica LEAVES IN Acinetobacter baumannii ISOLATED FROM SPUTUM SPECIMEN

Abstract

Antimicrobial properties of trace phytochemical compounds analysis of azadirachta indica leaves in acinetobacter baumannii isolated from sputum specimen. Azadirachta indica L. leaves was collected from Imo State University Farm, Owerri, Imo State. The aqueous and ethanolic extracts of the plants was extracted from 100g of dried powdered leaves with 100ml sterile distilled water and 100ml ethanol respectively using Soxhlet method. Fifty (50) sputum specimens was collected from patients admitted at Federal Medical Center Owerri for the isolation of Acinetobacter baumannii. Identification of Acinetobacter baumannii was done using morphological characteristics and biochemical test. Antimicrobial analysis of the characterized phytochemical compounds of Azadirachta indica leaves was tested against Acinetobacter baumannii using agar well diffusion. Two milliliters of each extract concentration was gently be mixed with the Mueller Hinton Agar and poured into Petri dishes to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts. Results shows Minimum Inhibitory Concentrations and Minimum Bactriocidal Concentration of Azadirachta indica extract against A. Baumanni showed No growth for at 25%, 50% and 100%. Qualitative phytochemical analysis on acetone and aqueous of Azadirachta indica leaves extracts showed the presence of Saponins, Flavonoid, Phenol, Alkaloid and Tannin. Antimicrobial effect of aqueous extract and ethanol of the Azadirachta indica leaves on extract on A. Baumannii showed no growth for 20%, 50% and 100% respectively.This study revealed the effects of aqueous extracts of Azadirachta indica leaf on Acinetobacter baumannii, as offer bacteria an attractive environment in which they can potentially flourish whereby causing significant damage if left untreated. While ethanol extract showed slightly turbid with growth.